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The optical density of the washed cells was measured and then adjusted to an OD600 of 0.1

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To avoid suspensions cells adhering to the plate, wells were washed with PBS

In 1989 a subcommittee of the United States Food and Nutrition Board, National Research Council, issued its last update on recommended dietary allowances (RDA) for protein and amino acids (These recommendations rely on N balance studies performed many years ago [24]

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